Figure 4

Monte Carlo sampling based flux balance analysis (FBA) simulations of the intervention sets identified by CCOpt and DetOpt for antibody production using the CHO cell model. Each panel shows a Monte Carlo distribution of FBA optimized target flux values, with the rows and columns corresponding to different caps on the number of interventions (L) and different optimization methods/settings, respectively. The x-axis represents the maximum antibody production rate in units of nmol/10⁶cells/day. The y-axis represents the sampled frequency of an FBA solution. The dashed lines denote the 5^th and 95^th percentile values, defined as the values below and above which 5 % of the data fall, respectively. A single dashed line indicates that these two percentile values are the same. The solid lines indicate the maximum production rates obtained using CCOpt or DetOpt.