Figure 1

Molecular composition of the stressosome. (A) The atomic model of the stressosome [10] is coloured by domain; C-terminal, RsbR-STAS domain is blue, N-terminal RsbR domain is yellow, RsbS is red and RsbT is not shown for clarity. (B) The stressosome as a two-dimensional network, with RsbR monomers (blue circles) connected by blue lines to display the distribution of RsbR dimers. Similarly, RsbS monomers (red stars) are connected by red lines to form dimers. Close contact between neighbouring proteins is represented by dashed lines. The numbers indicate the scheme we use to identify individual proteins in the structure. Three different neighbourhood configurations for RsbR are exemplified with the R1, R2, and R3 tags. (C) The four different neighbourhoods in the stressosome structure will experience different protein cooperativity effects on the phosphorylation reaction and thus different phosphorylation rates of the central protein. In the description of the neighbourhood composition, we always start by naming the unpaired protein; these are circled in the figure.