Figure 3

Model validation by simulating single-gene knockouts. A) Ime2 dynamics when deleting genes individually in the model. Red dashed line: rim11, ume6, ime1, and ime2 knockouts; red dotted curve: sok2 knockout; green curve: wild type. Mathematical models of single-gene knockouts are described in Additional file 2: Tables S1, S2, S3, S4 and S5. B) Pearson correlation between experimental sporulation/pre-sporulation ratios and simulated Ime2 values at steady state for five single-gene knockouts and wild type.