Figure 5
Simulation analyses of double-negative feedback loop between pSok2 and Ime1. A) Ime1 and Ime2 dynamics when varying c ime1 , the constant measuring half-maximum inhibition of Ime1 synthesis by pSok2. Red curve: c ime1  = ∞ (deleting the inhibition); cyan curve: c ime1  = 0.1 (decreasing the inhibition); blue curve: c ime1  = 0.001 (increasing the inhibition); green curve: c ime1  = 0.01 (baseline value). B) Ime1 and Ime2 dynamics when varying c sok2 , the constant measuring half-maximum inhibition of Sok2 phosphorylation by Ime1. Red curve: c sok2  = ∞ (deleting the inhibition); cyan curve: c sok2  = 0.5 (decreasing the inhibition); blue curve: c sok2  = 0.005 (increasing the inhibition); green curve: c sok2  = 0.05 (baseline value). C) Ime1 and Ime2 dynamics when varying c ime1 and c sok2 simultaneously. Red curve: c ime1  = ∞ and c sok2  = ∞ (deleting the feedback loop); cyan curve: c ime1  = 0.1 and c sok2  = 0.5 (decreasing the feedback loop); blue curve: c ime1  = 0.001 and c sok2  = 0.005 (increasing the feedback loop); green curve: c ime1  = 0.01 and c sok2  = 0.05 (baseline value). The mathematical model of the feedback knockout is described in Additional file 2: Table S6.