From: Computational modelling of the regulation of Insulin signalling by oxidative stress
Process | 2orate constant (#min-1) | Enzyme | Enz # | 1orate const (min-1) | PTM modifying rate | Rate MF | Reference |
---|---|---|---|---|---|---|---|
Synthesis (NULL → FOXO) | 0.0055 | E2F1 | 300 | 5.5 |  |  | |
Cytoplasm → nuclear transport | - | - | - | 0.1/0.55 = 0.182 |  |  | |
 |  |  |  |  | Akt-Phos Pa | 0.1 | [46] |
 |  |  |  |  | IKK-Phos Pd | 0.5 | |
 |  |  |  |  | JNK-Phos Pe | 10 | [44] |
Nuclear → cytoplasm transport | - | - | - | 0.1 × 0.55 = 0.055 |  |  | |
 |  |  |  |  | Akt-Phos Pa | 10 | [46] |
 |  |  |  |  | IKK-phos Pd | 10 | [47] |
 |  |  |  |  | JNK-Phos Pe | 0.1 | [44] |
Nuclear → DNAbound transport | - | - | - | 0.25 |  |  | [48] |
 |  |  |  |  | Akt-Phos Pa | 0.5 | [49] Figure 6B |
DNAbound → Nuclear transport | - | - | - | 0.25 × 0.5 = 0.125 |  |  | [48] |
basal phosphorylation | 5 × 10-5 |  |  |  |  |  |  |
basal dephosphorylation | 1 × 10-6 |  |  |  |  |  |  |
akt_phos_ factor | 6 | Â | Â | Â | Â | Â | Â |
ikk phos_factor | 3 | Â | Â | Â | Â | Â | Â |
jnk phos_factor | 2 | Â | Â | Â | Â | Â | Â |
FOXO → FOXO_Pa | akt phos_factor × basal phosphorylation | Akt | (104) | 0.5 |  |  | |
FOXO_Pa → FOXO | basal dephosphorylation | PP2A | 5 × 105 | 0.5 |  |  |  |
FOXO → FOXO_Pd | ikk phos_factor × basal phosphorylation | IKK | (104) | 0.5 |  |  | [47] |
FOXO_Pd → FOXO | basal dephosphorylation | PP2A | 5 × 105 | 0.5 |  |  |  |
FOXO → FOXO_Pe | jnk phos_factor × basal phosphorylation | JNK | (104) | 0.5 |  |  | [44] |
FOXO_Pe → FOXO | basal dephosphorylation | PP2A | 5 × 105 | 0.5 |  |  |  |
FOXO → FOXO_pUb | 1 × 10-6 | SCF/MDM2 | 103 | 10-3 |  |  | |
 |  |  |  |  | Akt (Pa) | 3 | |
 |  |  |  |  | IKK (Pd) | 22 | [47] Figure 5F |
Degradation (FOXO_pUb → NULL) | 1 × 10-4 | Proteasome | 103 | 0.1 |  |  |