Analysis of the miR-709 expression in the MHR close tothe MHB of the developing mouse embryo at E12. 5. (A)Representative sagittal section through an E12.5 CD-1 mouse embryo, hybridizedwith a radioactive mmu-miR-709 LNA oligonucleotide probe. At E12.5,miR-709 is strongly expressed in the ventral forebrain and midbrainand declines towards the ventral rostral hindbrain. (B) Shows the highmagnification of the ventral MHR region (delimited by the dashed red lines inA) and the 300 pixel long and 15 pixel wide region along which we analyzed theprofile of miR-709. The plot shows the grayscale values at each pointalong the region, calculated using the software ImageJ, and the expressionprofile α
(x) estimated usingleast-squares optimization. (C) Simulation of the extended MHB model forscenario ii). We choose the same parameter set used in Figure 2B right panels andβ
= 0 andκ = 20. For the simulation, the miRNA grayscaleprofile was normalized using the mean grayscale values of the light blue boxesin A. (D) Comparison of the Wnt1 expression profile in theoriginal and extended model for three diffusion coefficients for Fgf8(d5). Abbreviations: FB, forebrain; Mb, midbrain; MHB,mid-hindbrain boundary; Hb, hindbrain.