Skip to main content

Table 8 Synthetic lethality induced by elimination of phosphorylation/dephosphorylation reactions

From: Optimization and model reduction in the high dimensional parameter space of a budding yeast cell cycle model

Eliminated reaction

Impacted single mutation strains that are

 

viable (inviable) before (after) perturbation

Whi5 phosphorylationby Bck2

cln3 Δ, Multicopy BCK2, cdh1 Δ, sic1 Δ, swi5 Δ,

 

CLB5-db Δ, net1-ts, GAL-CLB2, APC-A

CKI phosphorylation by Cln2

bck2 Δ, GAL-SIC1, net1-ts, APC-A

CKI phosphorylation by Clb2

GAL-CLN3, cdh1 Δ, GAL-CLB5, CLB1 clb2 Δ

CKI dephosphorylation by Cdc14

bck2 Δ, cdh1 Δ, GAL-CLB2, APC-A

Whi5 phosphorylation by Cln3

bck2 Δ, cdh1 Δ, APC-A

SBF phosphorylation by Clb2

cdh1 Δ, CLB5-db Δ, APC-A

Whi5 phosphorylation by Cln2

bck2 Δ,APC-A

Whi5 dephosphorylation by Cdc14

APC-A

Net1 dephosphorylation by PPX

Multicopy CDC15

  1. Upon setting phosphorylation/dephosphorylation rate constants to zero (left column), viability is lost in several single mutation strains (right column).
Back to article page

BMC Systems Biology

ISSN: 1752-0509

Contact us