Figure 3

Gene expression levels of STAT1, IRF1 and SOCS1 after IFN-beta stimulation and the corresponding simulations from the ODE model. A-C) STAT1, IRF1 and SOCS1 RNA protein concentration in cell lysates were measured by RT-PCR in the Raw cells stimulated with IFN-beta (1000 units/ml); A) STAT1 RNA levels in the Raw cells stimulated with IFN-beta; B) IRF1 RNA levels in the Raw cells stimulated with IFN-beta; C) SOCS1 RNA levels in the Raw cells stimulated with IFN-beta. We plot here one out of three independent experiments, which were performed in duplicates. D-F) Model simulation and sensitivity analysis with ± 20% change for all parameters (shaded areas) for STAT1 (D), IRF1 (E), and SOCS1 (F) RNA levels.