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Figure 4 | BMC Systems Biology

Figure 4

From: Bridging the gap between gene expression and metabolic phenotype via kinetic models

Figure 4

Contribution of gene expression changes of individual reactions to 3-aminotriazole (3-AT) treatment tolerance. (A) Definition of the metric used to compare the effects of gene expression changes of individual reactions. The normalized tolerance change (NTC) for reaction i was defined as the average of the changes (Δ tol +i and Δ tol -i ) in the maximum 3-AT inhibition level (k his ) tolerated by S. cerevisiae in simulations where only the gene expression data (GED) of reaction i (g i ) are considered (No GED+g i ) or excluded (GED-g i ). The average was normalized by the difference in the maximum k his (Δ tol) in simulations with (GED) and without (No GED) gene expression changes for all reactions. In these simulations, kser→gly was varied linearly with k his such that when k his =1.0 then kser→gly=1.0 and when k his =4.87×10-1 then kser→gly=2.27×10-2. The values k his =1.0 and k his =0.0 represent no inhibition and complete inhibition, respectively. (B) Top ten reactions with the larger NTC magnitude. EC denotes the Enzyme Commission number. For lumped reactions (L), only the EC number for the first step is shown. (C) Overall gene expression changes for the ten reactions shown in B.

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