Figure 8
From: Bridging the gap between gene expression and metabolic phenotype via kinetic models
Contribution of gene expression changes of individual reactions to weak organic acid (WOA) treatment tolerance. (A) Definition of the metric used to compare the effects of gene expression changes of individual reactions. The normalized uptake change (NUC) for reaction i is defined as the average of the changes in the WOA uptake rate (Δ wur +i and Δ wur -i ) that reduced biomass to 5.0% of the reference value in simulations where only the gene expression data (GED) of reaction i (g i ) are considered (No GED+g i ) or excluded (GED-g i ). The average was normalized by the difference in the WOA uptake rate that reduced the biomass to 5.0% of the reference value in simulations with (GED) and without (No GED) gene expression changes for all reactions. (B) Normalized uptake changes for each WOA. We only show the reactions with 10 higher contributions. EC denotes the Enzyme Commission number. The y-axis shows only the EC number for the first step of lumped reactions (L). Note that the two most influential reactions, in bold font, were EC 2.7.1.1 and EC 4.1.1.1, for all cases.