Figure 1
From: Specific mutations in H5N1 mainly impact the magnitude and velocity of the host response in mice
MLD and phenotypical variables collected for all viruses at the 104PFU infection dosage. (A) Median lethal dose (MLD) values of the different viruses in 6-week-old BALB/c mice. MLD values for VN1203 and mutants were performed in one experiment (3 mice per inoculation dosage), and the previously calculated MLD for CA04 is included for comparison [22]. (B) Mice body weight measurements collected each day from 1 to 7 days post-infection for the 104 PFU infection dosages. For each virus and time point, the mean and the standard deviation of the mice body weight measurements are indicated by a vertical bar. (C), (D) and (E) Viral titer, viral messenger RNA, and viral genomic RNA measurements collected at 1, 2, 4 and 7 days post-infection for the 104 PFU infection dosages. For each virus and time point the set of individual measurements are indicated by filled dots and the mean amongst the individual samples is indicated by a horizontal bar. For the experiments shown in panels B-E, four to five mice were used for all VN1203-WT and pathogenicity mutant infections, while 3–4 mice were used for the CA04 infections. The same animals were used to derive all the phenotypical data (i.e. weight loss, virus titer and virus mRNA/genomic RNA levels).