Figure 2

GN-25 induces growth inhibition and suppresses spheroid formation in SNAIL-transduced HMLE models. (A) HMLE-SNAIL, Kras-HMLE and Kras-HMLE-SNAIL cells were exposed to different concentrations of GN-25 or Quercetin as + ve control as indicated and incubated for 72 hrs. Growth inhibition was evaluated using MTT assay. (B) Cells were allowed to grow as spheroids as described under Methods section. Once established, the spheroids were exposed to GN-25 (20 μM). Note: GN-25 suppresses growth and sphere-forming capacity of Snail-HMLEs. Figures are representative of three independent experiments.