The expression of EMT factors is changed by GN-25 treatment in Kras-HMLE-SNAIL cells. (A) Six EMT-associated genes were identified among genes that showed a significant LogRatio detection p-value in an Agilent two-color gene expression microarray (p ≤ .001). The heat map of Log2 gene expression ratios shows how many EMT-associated genes were up-regulated (red) or down-regulated (green) by treatment Kras-HMLE-SNAIL cells with GN-25 across four biological replicates. Genes with an asterisk (*) were among those shown to be uniquely regulated by GN-25 treatment in Kras-HMLE-SNAIL cells as determined by the ANOVA analysis that compared response to GN-25 treatment versus vehicle control in Kras-HMLE-SNAIL and Kras-HMLE cells (p ≤ .001, Benjamin-Hochberg FDR multiple test correction p ≤ .05). (B) The functional relationships for these genes and a substantial number of others linked to them from the Kras-HMLE-SNAIL-specific data set are mapped in the interaction network where the edges between genes represent data and peer-reviewed results contained in the Ingenuity Systems’ curated knowledgebase data set.