Global insights into energetic and metabolic networks in Rhodobacter sphaeroides

BMC Systems Biology

Table 3 Reactions predicted by iRsp1095 to be involved in NADPH generation

Gene identifier	Enzyme name	Reaction catalyzed*	EC number
RSP_0239 & RSP_0240	PntAB	NADP⁺ + NADH + 2 H⁺[p] = > NADPH + NAD⁺ + 2 H⁺	1.6.1.2
RSP_1939	NADPH-ferredoxin reductase	Reduced ferredoxin + NADP⁺ + H + <= > Oxidized ferredoxin + NADPH	1.18.1.2
RSP_2734	Zwf	D-Glucose 6-phosphate + NADP⁺ < = > 6PGL + NADPH	1.1.1.49
RSP_1217	Malic enzyme	(S)-Malate + NADP⁺ = > Pyruvate + CO₂ + NADPH	1.1.1.40
RSP_1593
RSP_0661	THF dehydrogenase	mlthf + NADP⁺ < = > methf + NADPH	1.5.1.5
RSP_0446	Icd	Isocitrate + NADP⁺ < = > 2-Oxoglutarate + CO₂ + NADPH	1.1.1.42
RSP_1559
RSP_1146 & RSP_1149	Glutamate synthase	2 L-Glutamate + NADP⁺ < = > L-Glutamine + 2-Oxoglutarate + NADPH + H⁺	1.4.1.13

*6PGL – D-Glucono-1,5-lactone 6-phosphate; mlthf – 5,10-Methylenetetrahydrofolate; methf – 5,10-Methenyltetrahydrofolate; THF dehydrogenase – 5,10-methylene-tetrahydrofolate dehydrogenase.

ISSN: 1752-0509