Simulation of pykF perturbation. The vertical axis in each graph shows the ratio of the concentration obtained for metabolites to the initial concentration of these metabolites or the ratio of enzymatic activity obtained to the initial enzymatic activity, while the horizontal axis shows the magnitude of perturbation. (A) Metabolites with a change rate between 0.5 and 2 fold. (B) Metabolites with a change rate between 0.1 and 10 fold; data for 3PG and PEP overlap. (C) Enzymatic activity change.