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Table 3 Characterization of the Rubrobacter xylanophilus UGTCase activity

From: Identifying reaction modules in metabolic pathways: bioinformatic deduction and experimental validation of a new putative route in purine catabolism

Reaction mixture composition Assay temperature Specific activityc(μmol min-1 mg-1)
25 mM potassium arsenate pH 6.8, 20 mM allantoate 37°C 0.046 ± 0.007 a
25 mM Hepes pH 6.8, 20 mM allantoate 37°C n.d. a
50 mM potassium phosphate pH 6.8, 20 mM allantoate, 5 mM ornithine, EcOTCase (100 units) 37°C 0.050 ± 0.002 b
25 mM Hepes pH 6.8, 20 mM allantoate, 5 mM ornithine, EcOTCase (100 units) 37°C n.d. b
50 mM potassium phosphate pH 6.8, 5 mM ornithine, EcOTCase (100 units) 37°C n.d. b
50 mM potassium phosphate pH 6.8, 20 mM allantoate, 5 mM ornithine, EcOTCase (100 units) 60°C 5.913 ± 0.584 b
50 mM potassium phosphate pH 6.8, 5 mM ornithine, EcOTCase (100 units) 60°C n.d. b
  1. aassessed by quantifying ureidoglycine.
  2. bassessed by quantifying citrulline.
  3. cData are the mean ± SD from at least three independent experiments. n.d., not detectable as defined in Methods.