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Table 2 Physiological parameters of reference and mutants in chemostat cultivation

From: Integrated analysis, transcriptome-lipidome, reveals the effects of INO- level (INO2 and INO4) on lipid metabolism in yeast

Strains µmax1 Left Glc (g/L) RQ YSX2 YSE3 YSG4 YSA5 YSS6 YSC7
RefC 0.384 ± 0.001 0.015 ± 0.000 0.817 ± 0.007 0.539 ± 0.010 n.d. 0.019 ± 0.001 n.d. n.d. 0.124 ± 0.003
Opi1CT 0.343 ± 0.001 0.009 ± 0.001 1.058 ± 0.020 0.557 ± 0.002 0.004 ± 0.000 0.019 ± 0.000 0.003 ± 0.000 n.d. 0.208 ± 0.004
Opi1CB 0.343 ± 0.001 0.009 ± 0.001 0.819 ± 0.003 0.550 ± 0.001 n.d. 0.019 ± 0.000 n.d. n.d. 0.106 ± 0.003
Ino2C 0.340 ± 0.000 0.018 ± 0.001 0.805 ± 0.011 0.597 ± 0.020 n.d. 0.018 ± 0.001 0.003 ± 0.000 n.d. 0.129 ± 0.006
Ino4C 0.369 ± 0.001 0.010 ± 0.000 0.837 ± 0.009 0.571 ± 0.007 n.d. 0.018 ± 0.002 n.d. n.d. 0.135 ± 0.002
Ino24C 0.436 ± 0.000 0.039 ± 0.001 0.889 ± 0.013 0.362 ± 0.028 0.098 ± 0.011 0.022 ± 0.003 0.019 ± 0.000 0.002 ± 0.000 0.101 ± 0.002
RefN 0.486 ± 0.000 20.387 ± 1.667 1.286 ± 0.008 0.128 ± 0.004 0.485 ± 0.048 0.008 ± 0.000 0.007 ± 0.001 0.002 ± 0.000 0.124 ± 0.004
Opi1N 0.499 ± 0.001 15.521 ± 1.809 1.260 ± 0.001 0.096 ± 0.003 0.316 ± 0.017 0.005 ± 0.000 0.005 ± 0.000 n.d. 0.075 ± 0.017
Ino2N 0.495 ± 0.001 28.286 ± 0.877 1.159 ± 0.008 0.168 ± 0.002 0.305 ± 0.015 0.009 ± 0.000 0.006 ± 0.001 n.d. 0.102 ± 0.005
Ino4N 0.494 ± 0.001 28.837 ± 0.427 1.261 ± 0.017 0.164 ± 0.003 0.337 ± 0.010 0.008 ± 0.000 0.006 ± 0.000 n.d. 0.103 ± 0.001
Ino24N 0.410 ± 0.000 35.944 ± 1.867 1.269 ± 0.010 0.058 ± 0.008 0.370 ± 0.026 0.017 ± 0.001 0.038 ± 0.001 n.d. 0.076 ± 0.005
  1. Note: All values are average ± SD from three biological replicates.
  2. 1 maximum specific growth rate on glucose during batch cultivation (with 10 g/L and 60 g/L initial glucose for C-limited and N-limited conditions, respectively)
  3. 2 Biomass yield on glucose in chemostat cultures (g biomass formed/g glucose consumed)
  4. 3 Ethanol yield on glucose in chemostat cultures (g ethanol formed/g glucose consumed)
  5. 4 Glycerol yield on glucose in chemostat cultures (g glycerol formed/g glucose consumed)
  6. 5 Acetate yield on glucose in chemostat cultures (g acetate formed/g glucose consumed)
  7. 6 Succinate yield on glucose in chemostat cultures (g succinate formed/g glucose consumed)
  8. 7 CO2 yield on glucose in chemostat cultures (mg CO2 formed/g glucose consumed)