Glycolysis inspired network. A) Schematic representation: The pathway consists in four enzymatic reactions with one branch where S
1 corresponds to the substrate, S
4 to the intermediate metabolites and S
5 to the product. B) Optimal profiles for the Glycolysis inspired pathway with inexhaustible substrate (single-objective case, Equation (25)), obtained with eSS. C) Resulting Pareto front for the minimization of the time and the enzyme cost needed to achieved a certain amount of product in a branched pathway coupled with enzyme dynamics using Equation (31). D) Optimal control profiles for enzyme activation for different points of the Pareto front, were approximated using ρ=120 steps with fixed duration. Enzyme and metabolite concentrations are expressed in concentration units (mM) and time in seconds (s).