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Table 3 Expression (Ct LSM) of each Rnf14 mRNA species and beta actin based on qRT-PCR of variant 1 and variant 3 transfected C2C12 cells

From: RNF14 is a regulator of mitochondrial and immune function in muscle

Treatments

All 3 endogenous and both constructs

All 3 endogenous

Variant 3 construct

Variant 1 construct

B actin

Control

24.99

26.59

31.93 (no amplification)

35.6 (no amplification)

23.16

Transfected variant 3

20.17

26.73

19.41

34.19 (no amplification)

23.36

Transfected variant 1

24.89

26.92

30.32 (no amplification)

29.19

23.10

  1. The standard error is 0.447 in all cases because we performed a system-wide normalization. ‘No amplification’ denotes multiple dissociation peaks i.e. non-specific amplification.