Skip to main content

Table 3 Expression (Ct LSM) of each Rnf14 mRNA species and beta actin based on qRT-PCR of variant 1 and variant 3 transfected C2C12 cells

From: RNF14 is a regulator of mitochondrial and immune function in muscle

Treatments All 3 endogenous and both constructs All 3 endogenous Variant 3 construct Variant 1 construct B actin
Control 24.99 26.59 31.93 (no amplification) 35.6 (no amplification) 23.16
Transfected variant 3 20.17 26.73 19.41 34.19 (no amplification) 23.36
Transfected variant 1 24.89 26.92 30.32 (no amplification) 29.19 23.10
  1. The standard error is 0.447 in all cases because we performed a system-wide normalization. ‘No amplification’ denotes multiple dissociation peaks i.e. non-specific amplification.