Network topology changes during GloSS-, NFAT- and NF-κB-assisted filtering. The immune response PPI network topological changes during the filtering process. Network measures were used to investigate the immunome interactome during filtering. The x-axis in each panel is the fraction of nodes removed during filtering. On the y-axis, the top panel shows changes in the network diameter, the middle panel changes in the average size of the isolated components excluding the largest or giant component (<s>). The bottom panel shows changes in the relative size of the largest or giant component (S). The relative size of the largest component is the number of nodes in the largest component divided by the number of nodes in the whole network. That is, n
= n/N, where n
is the relative size of the largest component, n is the number of nodes in the largest component and N is the number of nodes in the whole network). Each of the network measures were plotted against the fraction of links removed during filtering. The vertical line shows the point at which the paths between the TCR complex and the NF-κB and NFAT downstream components are broken. This also represents the point at which the filtering process stops. This indicates that the connectivity and robustness of the filtered network at this endpoint is maintained. Thus the connectivity and robustness inherent in the immunome interactome is maintained in the TPPIN.