Evaluation of the lower limit of Ade2 using TIPI-gTOW. (A) Growth of the cells harboring GFP-
ADE2 (YSM001) with the vector and the TEV plasmid on SC plates without the indicated amino acids. Six independent colonies of each strain were tested. (B) Copy numbers of the plasmids of GFP-
ADE2 (YSM001) in SC medium without the indicated amino acids. Four independent measurements were performed, and the average is shown. The error bar indicates the standard deviation. *: p < 0.01 for Student’s t-test.