Evaluation of the lower limits of Cdc20 protein on deletion of cell cycle regulators. (A) Relative copy number changes in the TEV plasmids in gene-deletion strains with GFP-
CDC20. Genes with previously identified genetic interactions with cdc20 mutation were boxed (summarized in Table 2). The original data used for the graph is shown in Table 1. (B) Relative copy number changes in TEV plasmids in gene-deletion strains with GFP-
CDC20 selected from A and simulation results. The degradation rate of kd20 is gradually increased until the cell cycle simulation indicates “dead.” In the simulation, 1: Clb1 and Clb2, 2: Clb5 and Clb6, 3: Cln1 and Cln2, and 4: Cln3 and Bck2 are implemented as single genes. 5: Swi6 is implemented into the component of SBF (together with Swi4) and MBF (together with Mbp1). The original data used for the graph is shown in Table 1 and Additional file 1: Table S2.