From: Evaluation of the lower protein limit in the budding yeast Saccharomyces cerevisiae using TIPI-gTOW
Name | Relevant characteristics | Source |
---|---|---|
pSBI40 | 2 μ ori, URA3, leu2d; plasmid for gTOW experiment | [7] |
pRS423ks | 2 μ ori, HIS3 | [11] |
pDS5 | p14*–TEV + | [22] |
pDS41 | GFP-TDegF-SF3b | [22] |
pSS1002 | hph-MX4, P_CDC19-GFP- TDegF ADE2 in pRS423ks | This study |
pSS1006 | P_CUP1-P14*–TEV + in pSBI40 | This study |
pSM001 | P_CDC19 -500 instead of P_CDC19 in pSS1002 | This study |
pSM002 | P_CDC19 -600 instead of P_CDC19 in pSS1002 | This study |