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Table 4 Plasmids used in this study

From: Evaluation of the lower protein limit in the budding yeast Saccharomyces cerevisiae using TIPI-gTOW

Name Relevant characteristics Source
pSBI40 2 μ ori, URA3, leu2d; plasmid for gTOW experiment [7]
pRS423ks 2 μ ori, HIS3 [11]
pDS5 p14*–TEV + [22]
pDS41 GFP-TDegF-SF3b [22]
pSS1002 hph-MX4, P_CDC19-GFP- TDegF ADE2 in pRS423ks This study
pSS1006 P_CUP1-P14*–TEV + in pSBI40 This study
pSM001 P_CDC19 -500 instead of P_CDC19 in pSS1002 This study
pSM002 P_CDC19 -600 instead of P_CDC19 in pSS1002 This study