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Table 4 Plasmids used in this study

From: Evaluation of the lower protein limit in the budding yeast Saccharomyces cerevisiae using TIPI-gTOW

Name

Relevant characteristics

Source

pSBI40

2 μ ori, URA3, leu2d; plasmid for gTOW experiment

[7]

pRS423ks

2 μ ori, HIS3

[11]

pDS5

p14*–TEV +

[22]

pDS41

GFP-TDegF-SF3b

[22]

pSS1002

hph-MX4, P_CDC19-GFP- TDegF ADE2 in pRS423ks

This study

pSS1006

P_CUP1-P14*–TEV + in pSBI40

This study

pSM001

P_CDC19 -500 instead of P_CDC19 in pSS1002

This study

pSM002

P_CDC19 -600 instead of P_CDC19 in pSS1002

This study