The influence of threshold c on sensitivity and specificity of QuartetS for two simulated data. The sensitivity and specificity plotted for all 100 runs with the horizontal line in the bar indicating the median performances. The upper whisker extends from the hinge to the highest value that is within 1.5×I Q R of the hinge, where IQR is the inter-quartile range, or distance between the first and third quartiles. The lower whisker extends from the hinge to the lowest value within 1.5×I Q R of the hinge. Data beyond the end of the whiskers are outliers and plotted as solid squares.