A bioinformatics approach reveals novel interactions of the OVOL transcription factors in the regulation of epithelial – mesenchymal cell reprogramming and cancer progression

Table 10 AP1 (FOS and JUN) with MYC peak occupancy in the OI-MET gene set, based on ChIPBase datasets

JUN	Peak occupancy (# of JUN and MYC peaks overlapping promoters, of 4, 102 promoters)
	MET
	Only JUN	Only MYC	Both expected	Both observed	Fold change	P -value
Peaks overlapping promoters	796	305	0.43	152	350.51	<0.0001**
Peaks	120679	19030
	Non- MET
	Only JUN	Only MYC	Both expected	Both observed	Fold change	P- value
Peaks overlapping promoters	757	910	0.31	670	2145.60	<0.0001**
Peaks	112929	80131
FOS	Peak occupancy (# of FOS and MYC peaks overlapping promoters, of 4, 102 promoters)
	MET
	Only FOS	Only MYC	Both expected	Both observed	Fold change	P- value
Peaks overlapping promoters	34	305	0.68	17	24.96	<0.0001**
Peaks	3282	19030
	Non- MET
	Only FOS	Only MYC	Both expected	Both observed	Fold change	P- value
Peaks overlapping promoters	251	910	0.31	253	804.10	<0.0001**
Peaks	37162	80131

Peak occupancy is significantly enriched for both JUN and FOS with MYC, for both the MET and Non-MET models, relative to the expected if they were independent. This result is consistent with cooperative regulation of a subset of these promoters by AP1 and MYC. (**Significant at 0.0001 P-value).

ISSN: 1752-0509