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Table 2 Regulation of AP1, STAT1, STAT3, and NFKB expression by OVOLS

From: A bioinformatics approach reveals novel interactions of the OVOL transcription factors in the regulation of epithelial – mesenchymal cell reprogramming and cancer progression

    BC fold change PC fold change
  Gene Expression level OVOL1 OVOL2 OVOL1&2 Up/Down switch OVOL1 OVOL2 OVOL1&2 Up/Down switch
AP1 FOS (c- Fos) Gene 1.4 1.4 1.4   1.0 0.8 1.0  
FOSB Gene 1.0 2.1 1.1 Up 0.7 0.5 0.4 Down
  NM _006732 2.6 0.0 0.0 Switch 10.0 10.0 10.0 Switch
  NM _001114171 0.9 2.3 1.1 0.7 0.5 0.4
FOSL1 (Fra- 1) Gene 0.9 0.9 0.8   1.1 1.0 0.7  
FOSL2 (Fra- 2) Gene 0.7 0.7 0.6 Down 0.8 1.2 1.1  
JUN (c- Jun) Gene 1.0 0.9 0.8   0.5 0.5 0.5 Down
JUNB Gene 1.5 2.2 2.1 Up 1.2 1.9 1.4 Up
JUND Gene 1.1 1.1 1.3   1.2 1.6 1.2 Up
STAT STAT1 Gene 1.1 1.3 1.0   1.0 1.4 2.0 Up
  NM _007315 1.1 1.3 1.0   1.1 1.4 1.9 Up
  NM _139266 1.2 1.3 1.1   0.8 1.7 2.2 Up
STAT3 Gene 0.9 1.2 0.9   0.0 0.4 6.3 Up/ down
  NM _139276 0.5 1.6 0.9 Up/ down 0.0 0.4 0.0 Switch
  NM _003150 0.8 1.2 0.9   0.0 1.0 10.0
  NM _213662 1.0 1.2 0.9   0.1 1.2 8.6 Up/ down
NFKB NFKB1 (NFkB1) Gene 1.0 1.3 1.2   1.0 1.1 1.0  
  NM _003998 1.0 1.3 1.2   1.3 1.1 1.2  
  NM _001165412 1.0 1.2 1.2   0.8 1.0 0.9  
NFKB2 (NFkB2) Gene 0.9 1.5 1.2 Up 1.4 1.7 0.8 Up /down
  NM _001077494 0.9 1.5 1.0 Up 1.9 3.8 0.8 Up
  NM _002502 0.7 1.6 0.9 Up 1.3 1.9 0.7 Up
  NM _001261403 0.9 1.4 0.9   1.4 1.4 0.8  
REL (c- Rel) Gene 1.2 1.3 1.1   1.6 2.2 1.6 Up
RELA Gene 1.0 1.1 1.1   0.9 0.9 0.8  
  NM _021975 1.0 1.0 1.0   1.0 1.0 1.0  
  NM _001145138 1.0 1.1 1.1   0.9 0.9 0.8  
RELB Gene 0.8 1.5 1.1 Up 1.3 2.4 1.0 Up
  1. We assessed the effects of the OVOLs on expression of AP1, STAT1, STAT3, and NFKB based on our RNA-Seq data for both BC and PC. We looked for up-regulation (at least one observation of Fold Change ≥ 1.5), down-regulation (at least one observation of FC ≤ 0.67), or isoform switching (switch). Within the AP1 (FOS/JUN) group, the FOS genes are regulated in both BC and PC, at both the gene and isoform levels (e.g. FOSB). The individual members of the JUN family are regulated at the gene level, similar to the way isoform switching is used in the FOS group. Within the STAT group (Stat1/Stat3), isoform level regulation is employed in BC while both isoform switching and gene level regulation are employed in PC. Within the NFKB group, gene level regulation is employed.