A bioinformatics approach reveals novel interactions of the OVOL transcription factors in the regulation of epithelial – mesenchymal cell reprogramming and cancer progression

Table 8 AP1 (FOS and JUN) peak occupancy in the OI-MET gene set, based on ChIPBase datasets

	Peak occupancy (number of peaks overlapping with promoters)
JUN
	Not _cancer	MET
Peaks overlapping promoters	287	796
Peaks	75474	120679	Fold change	P- value
Peak occupancy rate	0.38%	0.66%	1.73	<0.0001**
	Not _cancer	Non- MET
Peaks overlapping promoters	287	757
Peaks	75474	112929	Fold change	P- value
Peak occupancy rate	0.38%	0.67%	1.76	<0.0001**
	MET	Non- MET
Peaks overlapping promoters	796	757
Peaks	120679	112929	Fold change	P- value
Peak occupancy rate	0.66%	0.67%	1.02	0.7773
FOS
	Not _cancer	MET
Peaks overlapping promoters	103	34
Peaks	20695	3282	Fold Change	P- value
Peak occupancy rate	0.50%	1.04%	2.08	0.0003**
	Not _cancer	Non-MET
Peaks overlapping promoters	103	251
Peaks	20695	37162	Fold Change	P- value
Peak occupancy rate	0.50%	0.68%	1.36	0.0105*
	MET	Non-MET
Peaks overlapping promoters	34	251
Peaks	3282	37162	Fold Change	P- value
Peak occupancy rate	1.04%	0.68%	0.65	0.0252*

Peak occupancy is significantly increased for both JUN and FOS, in both the MET and Non-MET models, relative to the non-cancer model. The effect is essentially the same for JUN in the MET and Non-MET models, but the effect for FOS is greater in the MET model. These results are consistent with both JUN and FOS transcription factors trans-locating to occupy the OI-MET promoters in both MET and non-MET models. (*Significant at 0.05 P-value. **Significant at 0.001 P-value).

ISSN: 1752-0509