A bioinformatics approach reveals novel interactions of the OVOL transcription factors in the regulation of epithelial – mesenchymal cell reprogramming and cancer progression

Table 9 AP1 (FOS and JUN) with MYC promoter occupancy in the OI-MET gene set, based on ChIPBase datasets

JUN	Promoter occupancy (# promoters overlapping both JUN and MYC peaks, from a total of 4, 102 promoters)
	MET
	Only JUN	Only MYC	Both expected	Both observed	Fold change	P- value
Promoters with one or more peaks	503	436	53.46	228	4.26	<0.0001**
	Non- MET
	Only JUN	Only MYC	Both expected	Both observed	Fold change	P- value
Promoters with one or more peaks	483	398	46.86	288	6.15	<0.0001**
FOS	Promoter occupancy (# promoters overlapping both FOS and MYC peaks, from a total of 4, 102 promoters)
	MET
	Only FOS	Only MYC	Both expected	Both observed	Fold change	P- value
Promoters with one or more peaks	43	436	4.57	22	4.81	<0.0001**
	Non- MET
	Only FOS	Only MYC	Both expected	Both observed	Fold change	P- value
Promoters with one or more peaks	161	398	15.62	100	6.40	<0.0001**

Promoter occupancy is significantly enriched for both JUN and FOS with MYC, for both the MET and Non-MET models, relative to the expected if they were independent. This result is consistent with cooperative regulation of a subset of these promoters by AP1 and MYC. (**Significant at 0.0001 P-value).

ISSN: 1752-0509