Figure 2

Optimization for a high expression condition results in eGFP expression exceeding the wild-type. In addition to wild-type eGFP, eight variants were generated. The high expression variants were made from a codon usage table (HT) and matrix (H1-H3) constructed using the 100 most highly expressed genes in yeast grown in rich media. Control variants were constructed from the standard usage table (CT) and control matrix (C1-C3). eGFP protein expression was measured using flow cytometry for yeast grown in YPD. Biological triplicates were used to calculate standard deviations, indicated by error bars and p-values were calculated using a t-test to determine statistical significance (described in text of paper).