Figure 5
From: Analysis of Wnt signaling β-catenin spatial dynamics in HEK293T cells
Confocal 3D quantitation technique validation with quantitative Western blot for HEK293T Whole Cell Lysates. Western blots for β-catenin:(A) Wnt3A stimulation; (B) CHX inhibition for 0, 1, 2 or 4 hours; (C) Comparison between the results of confocal quantification and Western blot validation. P-values for both Wnt3A and CHX are greater than p = 0.05. (Error Bars: Standard deviation. Selected blots showing non-continuous lanes, replicates are labeled as A or B with β-tubulin used as a loading control.)