Figure 7

HEK293T cell model simulations, optimization and results. Computational optimization of Model 1 for the total β-catenin data obtained using quantitative confocal microscopy (shown in Figure 5). Specifically, (A) the model was fitted to the degradation (cycloheximide inhibition experiments in red circles) and Wnt perturbation (blue diamonds) dynamics of total β-catenin. Results show a reasonable correlation between Model 1 and the experiment. (B) Redistribution of Wnt signaling pathway proteins and the interacting protein complexes during the steady state using the optimized whole cell Model 1 (log scale). It should be noted that concentrations of protein and complexes in the degradation complex (in red box) are significantly lower than the other proteins (and complexes) in the model.