Exposure time effects on single gene (rank) analysis. (A) SSEM-Lasso ranks of FL’s primary gene target, ERG11 (squares), were compared to gene rank averages for six orthogonal genes, MPS1, ADE13, TOP2, CDC9, PAB1 and UBA1 (circles), across increasing ETs. Error bars represent standard deviation for orthogonal gene ranks. (B) SSEM-Lasso ranks of all FL targets, ERG11 (squares), ERG6 (triangles), UPC2 (hexagons) and HAP1 (crosses) versus FL ET experiments. Cells were treated with FL concentrations that corresponded to increasing growth inhibitory percentages, GI%s (x-axis). Lower ranks indicated better prediction of FL action on an individual gene. All ranks were the averages of two replicates.