Concentration effects on single gene (rank) analysis. (A) SSEM-Lasso ranks of FL’s primary gene target, ERG11 (diamonds), were compared to gene rank averages for six orthogonal genes, MPS1, ADE13, TOP2, CDC9, PAB1 and UBA1 (circles), across increasing FL concentrations. Error bars represent standard deviation for orthogonal genes. (B) SSEM-Lasso ranks of all FL targets, ERG11 (diamonds), ERG6 (triangles), UPC2 (hexagons) and HAP1 (crosses) versus concentration experiments. Cells were treated with FL concentrations that corresponded to increasing growth inhibitory percentages, GI%s (x-axis). Lower ranks indicated better prediction of FL action on an individual gene. All ranks are the averages of two replicates.