Figure 2

Selected plasma metabolites having a significant correlation with polyp counts in association with the Genotype by Diet interaction effect. The nine metabolites were selected from Table 3 of significant plasma metabolites having a correlation with polyp counts in association with a Genotype by Diet interaction effect. They reflects how the combination of ApcMin/+ mutation and high fat diets is associated with the plasma metabolome and translates into intestinal polyps formation. In all subplots A-I, the correlation p-values (pGLM) were obtained from fitting the GLM after adjustment for multiplicity (‘see ‘Methods’ and Table 3). To graphically show the grouping and localization of the samples as well as to visualize the linearity and correlation at play for each significant metabolite, the linear regression line is plotted (dotted lines) with its corresponding determination coefficient (r2) and the Pearson correlation coefficient (ρ). Because of the vertical alignment of all sample points from the WT-LF and MU-LF groups, the resulting coefficient of determination (r2) is 0 and the Pearson correlation coefficient (ρ) is mathematically undetermined. In contrast, for all the other samples in the MU-LF and MU-HF experimental groups, where r2 and ρ are both meaningful, we observe for all metabolites (A-I) the best coefficient of determination (r2) and the largest Pearson correlation coefficient (ρ) in the MU-HF group (blue) in comparison to the MU-LF group (green). Hippuric acid (A), Pyrophosphate (B), Nicotinamide (C), Glycine (D), Phenylalanine (E), Methionine (F), Tryptophane (G), Threonine (H), Glutamic acid (I) for all combinations of Genotype and Diet factors. WT-LF, WT-HF, MU-LF, and MU-HF stand respectively for the following groups: Apc Wild-Type - Low Fat Diet, Apc Wild-Type - High Fat Diet, Apc Mutant - Low Fat Diet, Apc Mutant - High Fat Diet. Concentration levels are normalized on a transformed scale as explained in the ‘Methods’ section.