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Table 4 Comparison of experimental anaerobic glucose-limited chemostat growth data with EcoCyc–18.0–GEM and iJO1366 constraint-based model predictions

From: A genome-scale metabolic flux model of Escherichia coli K–12 derived from the EcoCyc database

Anaerobic fluxes Experimental EcoCyc iJO1366
Specific growth rate µ (h r -1) 0.30 0.24 0.24
Glucose uptake (mmol/g CDW /hr) 10.0 10.00 10.00
O 2 uptake 0.00 0.00 0.00
NH 4 uptake   2.52 0.82
Sulfate uptake   0.06 0.06
Phosphate uptake   0.23 0.22
CO 2 production   0.04 -0.08
H 2O production   -1.92 -1.84
H + production 27.8 27.5  
Acetate production 7.5 8.29 8.23
Formate production 11.3 17.37 17.25
Succinate production 1.2 0.00 0.08
Ethanol production 8.7 8.13 8.08
  1. Metabolite uptake and production rates are in units of mmol/gCDW/hr. Growth is in units of hr -1. Experimental data from [24] via [25]. Formate-hydrogen lyase (FHLMULTI-RXN) was left inactive for purposes of comparison.