Exploring metabolism flexibility in complex organisms through quantitative study of precursor sets for system outputs

Table 6 Minimum and maximum utilization of input in each output

(Ctrl) treatment
Input		Glucose		Glycerol		Acetate		BHBA		Lysine		Threonine¹		Isoleucine¹		Leucine¹		Valine¹		Histidine¹		Arginine¹		Alanine¹		Glutamine¹
		1422		17,5		1020		336		16,1		1,40		13,1		12,1		12,7		1,38		26,4		9,33		6,10
Output		Minimum and maximum utilisation of Input in each output (in mmol/h/half udder of Carbon)
		Min	Max	Min	Max	Min	Max	Min	Max	Min	Max	Min	Max	Min	Max	Min	Max	Min	Max	Min	Max	Min	Max	Min	Max	Min	Max
Glycerol3P	98.9	32.9	97.0	1.24	12.5	0	38.4	0	16.0	0	1.1	0	0.1	0	1.3	0	1.0	0	1.2	0	0.1	0	2.0	0	1.0	0	0.6
Lactose	886	751	886	0	7.7	0	79.8	0	33.2	0	2.3	0	0.1	0	2.6	0	2.0	0	2.4	0	0.2	0	4.0	0	2.0	0	1.1
C4	40.3	0		0		0		40.3		0		0		0		0		0		0		0		0		0
c6	27.1	0		0		18.0		9.0		0		0		0		0		0		0		0		0		0
c8	17.8	0		0		13.4		4.5		0		0		0		0		0		0		0		0		0
c10	46.6	0		0		37.3		9.3		0		0		0		0		0		0		0		0		0
c12	50.8	0		0		42.3		8.5		0		0		0		0		0		0		0		0		0
c14	195	0		0		167		27.8		0		0		0		0		0		0		0		0		0
c16	301	0		0		263		37.6		0		0		0		0		0		0		0		0		0
Glycine ²	10.0	3.5	8.0	0.1	0.9	1.0	3.7	0.4	1.5	0.0	0.1	0.1		0.0	0.1	0.0	0.1	0.0	0.1	0.0	0.1	0.0	0.2	0.0	0.1	0.0	0.1
Glutamate ²	2.7	0.0	1.1	0.0	0.1	1.0	1.6	0.4	0.7	0.0	0.1	0.0	0.1	0.0	0.1	0.0	0.1	0.0	0.1	0.0	0.1	0.0	0.1	0.0	0.1	0.0	0.1
Proline ²	53.3	0.7	20.8	0.0	0.7	20.0	32.4	8.3	13.5	0.5	0.7	0.0	0.1	0.4	0.9	0.5	0.8	0.3	0.7	0.0	0.1	1.0	1.8	0.2	0.6	0.3	0.5
Aspartate ²	13.7	0.2	7.4	0.0	0.3	3.8	8.2	1.6	3.4	0.1	0.2	0.0	0.1	0.1	0.3	0.1	0.2	0.1	0.3	0.0	0.1	0.2	0.4	0.0	0.2	0.0	0.1
Serine ²	21.6	7.0	20.6	0.3	2.6	0.0	8.2	0.0	3.4	0.0	0.2	0.4	0.4	0.0	0.3	0.0	0.2	0.0	0.3	0.2	0.3	0.0	0.4	0.0	0.2	0.0	0.1
CO2	1126	396	565	1.3	14.5	343	446	142	185	12.1	15.3	0.7	0.8	9.1	12.4	8.7	11.3	9.1	12.1	0.7	1.0	15.1	20.3	6.3	8.8	4.2	5.6

A local-search algorithm allowed us to compute the minima and maxima of each AIO coefficient for the two treatments (Ctrl), (CN) (in mmol/h/half udder of Carbon). These tables allow discriminating the response of the mammary gland to the two treatments without requiring selection of a flux distribution for reactions in the metabolic network. (CN) treatment (protein intake by food) is characterized by a lower proportion of glucose which is oxidized in CO2 than in (Ctrl).
⁽¹⁾Amino acid input corresponded to positive balances between amino acid net uptake and amino acid and utilization in milk protein (i.e. peptide output).
⁽²⁾Amino acid Output corresponded to negative balance between, amino acid net uptake and utilization in milk protein (i.e. peptide output).

ISSN: 1752-0509