|
(CN) treatment
|
|---|
|
Input
|
Glucose
|
Glycerol
|
Acetate
|
BHBA
|
Lysine1
|
Isoleucine1
|
Leucine1
|
Valine1
|
Arginine1
|
Glutamine1
|
|---|
| | |
1392
|
17.2
|
924
|
668
|
21.5
|
21.4
|
22.6
|
19.3
|
26.9
|
8.95
|
|---|
|
Output
|
Minimum and maximum utilisation of input in each output (in mmol/h/half udder of Carbon)
|
| | |
Min
|
Max
|
Min
|
Max
|
Min
|
Max
|
Min
|
Max
|
Min
|
Max
|
Min
|
Max
|
Min
|
Max
|
Min
|
Max
|
Min
|
Max
|
Min
|
Max
|
|
Glycerol3P
|
117
|
36.1
|
115
|
1.8
|
11.3
|
0
|
22.8
|
0
|
44.5
|
0
|
1.6
|
0
|
2.3
|
0.0
|
2.0
|
0.0
|
2.0
|
0.0
|
2.2
|
0.0
|
0.9
|
|
Lactose
|
1002
|
866
|
1002
|
0
|
9.2
|
0
|
37.9
|
0
|
74.0
|
0
|
2.6
|
0
|
3.9
|
0.0
|
3.3
|
0.0
|
3.3
|
0.0
|
3.7
|
0.0
|
1.5
|
|
C4
|
46.4
|
0
|
0
|
0
|
46.4
|
0
|
0
|
0
|
0
|
0
|
0
|
|
C6
|
33.5
|
0
|
0
|
22.3
|
11.2
|
0
|
0
|
0
|
0
|
0
|
0
|
|
C8
|
23.0
|
0
|
0
|
17.2
|
5.7
|
0
|
0
|
0
|
0
|
0
|
0
|
|
C10
|
64.6
|
0
|
0
|
51.7
|
12.9
|
0
|
0
|
0
|
0
|
0
|
0
|
|
C12
|
73.4
|
0
|
0
|
61.2
|
12.2
|
0
|
0
|
0
|
0
|
0
|
0
|
|
C14
|
250
|
0
|
0
|
215
|
35.8
|
0
|
0
|
0
|
0
|
0
|
0
|
|
C16
|
343
|
0
|
0
|
300
|
42.9
|
0
|
0
|
0
|
0
|
0
|
0
|
|
glycine 2
|
6.9
|
2.1
|
5.5
|
0.0
|
0.5
|
0.4
|
1.3
|
0.7
|
2.6
|
0.0
|
0.1
|
0.0
|
0.1
|
0.0
|
0.1
|
0.0
|
0.1
|
0.0
|
0.1
|
0.0
|
0.1
|
|
Alanine 2
|
9.8
|
1.4
|
9.6
|
0.0
|
0.9
|
0
|
2.4
|
0
|
4.7
|
0
|
0.2
|
0
|
0.3
|
0
|
0.2
|
0
|
0.2
|
0
|
0.2
|
0
|
0.1
|
|
glutamate 2
|
31.7
|
0.8
|
8.8
|
0.0
|
0.4
|
6.7
|
9.0
|
13.0
|
17.6
|
0.4
|
0.5
|
0.4
|
0.7
|
0.6
|
0.8
|
0.3
|
0.5
|
0.7
|
0.9
|
0.3
|
0.4
|
|
proline 2
|
55.0
|
1.4
|
15.3
|
0.0
|
0.6
|
11.6
|
15.6
|
22.6
|
30.5
|
0.7
|
0.9
|
0.8
|
1.3
|
1.0
|
1.4
|
0.5
|
0.9
|
1.2
|
1.6
|
0.5
|
0.7
|
|
aspartate 2
|
16.5
|
0.6
|
7.0
|
0.0
|
0.3
|
2.7
|
4.5
|
5.3
|
8.9
|
0.2
|
0.3
|
0.3
|
0.5
|
0.2
|
0.4
|
0.2
|
0.4
|
0.3
|
0.5
|
0.1
|
0.2
|
|
serine 2
|
22.5
|
6.9
|
22.0
|
0.3
|
2.2
|
0
|
4.4
|
0
|
8.5
|
0
|
0.3
|
0
|
0.4
|
0
|
0.4
|
0
|
0.4
|
0
|
0.4
|
0
|
0.2
|
|
CO2
|
1021
|
244
|
402
|
2.0
|
12.5
|
180
|
227
|
351
|
444
|
16.3
|
19.8
|
14.2
|
19.1
|
15.8
|
20.0
|
13.4
|
17.4
|
14.8
|
19.2
|
5.9
|
7.8
|
- A local-search algorithm allowed us to compute the minima and maxima of each AIO coefficient for the two treatments (Ctrl), (CN) (in mmol/h/half udder of Carbon). These tables allow discriminating the response of the mammary gland to the two treatments without requiring selection of a flux distribution for reactions in the metabolic network. (CN) treatment (protein intake by food) is characterized by a lower proportion of glucose which is oxidized in CO2 than in (Ctrl).
-
(1)Amino acid input corresponded to positive balances between amino acid net uptake and amino acid and utilization in milk protein (i.e. peptide output).
-
(2)Amino acid Output corresponded to negative balance between, amino acid net uptake and utilization in milk protein (i.e. peptide output).
