Figure 5

Confirmation of selected genes by qRT-PCR. TGRL lipolysis products (TL) compare to Media (M) alone treatment. Genes related to (A) Transcription factors, (B) Pro-inflammatory Response, (C) Cell cycle and Apoptosis, D) Metabolism and Signalling pathway. HBMVEC were treated with either Media (M) or TGRL lipolysis products (TL) for 3 hr. Microarray analysis: (pooled n = 3 per GeneChip as compared to TGRL-treated group). For qRT-PCR, the expression of each gene was normalized to that of GAPDH and then fold change was calculated as a ratio of expression after lipolysis products treatment relative to TGRL controls (individual n = 3). An asterisk (*) denotes p-value ≤ 0.05.