Fit of the "Control(+)" data. After fixing the rates µ, λ, ν from the fitting of the negative decay pattern in Figure 4, we use the network in Figure 10 in order to fit the decay pattern when all three factors, miRNA, NOT1 and PAN3 are expressed. The values of the rates are λ
= 0.1501 −1 and µ
= 0.0493 min−1 with 95% confidence intervals [0.0908, 0.2094] and [0.0373, 0.0612], respectively. These rates indicate that when both NOT1 and PAN3 are expressed together with the miRNA, about 84% of the mRNA in the experiment are degraded by the action of miRISC.