Cooperative effect of PAN3. Percent of mRNA at steady state expression level in each of the three main states of the networks in Figures 9 and 11. State 0 represents the mRNAs that are not bound to miRISC and not bound to the competing alternative complexes. The alternative degradation pathways lead to state 1 whereas the miRISC-mediated pathway leads to state 2. The blue histogram refers to the case when only the miRNA and NOT1 are expressed whereas the histogram in red refers to the experiments when all three factors miRNA, NOT1 and PAN3 are expressed. The percentage of mRNA in state 2 is strongly increased by the expression of PAN3 at the expenses of the amount of mRNA in state 1. Since the rates µ
do not differ, this shift in the amount of mRNA between the states is due to a strong increase in the rate λ
compared to λ
. This indicates that the most important role of PAN3 is to shift the balance towards miRNA-mediated decay.