Modeling the dependence of respiration and photosynthesis upon light, acetate, carbon dioxide, nitrate and ammonium in Chlamydomonas reinhardtiiusing design of experiments and multiple regression

Table 5 Randomly-generated combinations of factors and associated mean experimental responses used for experimental validation

	[Ac.]	Light	[NH₄ ⁺]	[NO₃ ^-]	[CO₂]	CR	MA_CYT	MA_ALT	ΦPSII₈₀₀	NPQ₈₀₀	P₈₀₀
1	0.58	61	10.0	7.0	1.5	8.1 ± 0.5	7.3 ± 0.2	10.1 ± 0.2	0.273 ± 0.019	0.218 ± 0.020	50.3 ± 1.7
2	0.32	58	15.0	3.0	1.5	6.7 ± 0.3	5.8 ± 0.3	6.6 ± 0.4	0.250 ± 0.025	0.183 ± 0.028	58.9 ± 2.4
3	0.21	90	15.0	9.0	1.5	6.4 ± 0.5	6.1 ± 0.2	8.0 ± 1.0	0.369 ± 0.005	0.235 ± 0.052	64.0 ± 0.4
4	0.75	65	12.0	5.0	1.5	10.6 ± 0.8	8.8 ± 0.6	8.3 ± 0.8	0.307 ± 0.064	0.166 ± 0.075	48.8 ± 1.6
5	0.35	144	0.0	10.0	1.5	9.0 ± 1.3	8.3 ± 0.8	12.9 ± 0.9	0.428 ± 0.013	0.244 ± 0.042	134.9 ± 9.1
6	0.25	75	7.5	10.0	1.5	5.2 ± 0.7	5.2 ± 0.7	7.2 ± 0.6	0.270 ± 0.022	0.273 ± 0.025	71.3 ± 3.9
7	0.82	167	0.0	0.0	1.5				0.467 ± 0.054	0.097 ± 0.021	15.2 ± 3.2
8	1.00	21	15.0	0.0	1.5				0.260 ± 0.015	0.133 ± 0.024	23.2 ± 1.9

Data result from 3 independent sets of cultures and measurements. [Ac.], acetate concentration.

ISSN: 1752-0509