Pegasus fusion annotation flow. For each phase, the figure shows how the feature vector is constructed on the left side of the panel. In Fusion Detection Tools Candidates Integration step, report files from several fusion detection tools are loaded in a unique fusion database. In Chimeric Transcript Sequence Reconstruction and Functional Analysis phase, the fusion transcript is assembled according to the fusion breakpoint coordinates, the reading frame is checked and the protein domain annotation is performed on the resulting fused sequence. Finally, the Driver Fusion Prediction applies machine learning techniques to determine prediction scores.