OVCAR5 proliferation was dependent on both cell density and IGF1 dose. A, OVCAR5 exhibited concentration-dependent proliferation in response to IGF1 treatment at all three cell densities (31,000, 67,000, 126,000 cells/well); however, the extent of proliferation induced by a set concentration of IGF1 treatment was different at the three cell densities. B, Treatment dose (i.e., pmol of IGF1) impacted the extent of OVCAR5 proliferation while concentration had minimal effect. OVCAR5 were plated at a fixed density (116,000 cells/well) to control for cell confluency, and treatment volumes were varied to result in two doses of IGF1 at three different concentrations. *indicates significantly different (p < 0.05) between doses for each concentration, n = 3 per treatment.