Figure 5

Activated β 1 integrin, detected by 9EG7, in MG-63 osteoblasts on micro-pillars. On the planar surface (left) activated β 1 integrins are homogeneously distributed, whereas on the micro-pillared surface (right) activated β 1 integrins are partly clustered on top of the pillars (arrows). Note that activated β 1 integrins are not clustered solely on pillars but formed also aggregates in parts in between the pillars (arrowhead). Confocal microscopy (LSM 780, Carl Zeiss, bars = 10 μm upper row, 2 μm lower row).