Membrane related dynamics and the formation of actin in cells growing on micro-topographies: a spatial computational model

Table 2 Parameters and results of simulation runs shown in Figure 9

Figure 9 panel		Top left	Top right	Bottom left	Bottom right
rCofRegAppearance		2.0	2.0	0.5	0.5
rCofRegDisappearance		0.5	0.5	2.0	2.0
Angle deviation		$N (0, {1 5^{°}}^{2})$	$N (0, 15^{2})$	$N (0, 30^{2})$	$N (0, 30^{2})$
Cofilin $_{/ total}^{active}$	on pillars	0.028	0.021	0.22	0.26
Cofilin $_{/ total}^{active}$	between pillars		0.141		0.38
Average filament length (# particles)		21.1	18.5	13.0	11.4
Maximum filament length (# particles)		69	58	29	33

All four runs had these parameter values in common: initial actin amount 500 (changes here did not significantly change the results as most actin was “produced” during the simulation), integrin amount 200 (a lower amount, 100, lead to 20% longer filaments in situations where cofilin activity was low, ie corresponding to the top panels), cofilin amount 800 (lower values lead slightly longer filaments in all four settings).

ISSN: 1752-0509