Figure 1

Flow chart describing the construction of the zebrafish intracellular PPI network. One-way ANOVA was applied to select the target protein pools. PPIs of Homo sapiens data were obtained from the BioGRID database, and orthologous information between zebrafish and H. sapiens was extracted from the InParanoid database. By using a dynamic model, we were able to construct the candidate PPI networks for zebrafish. As these candidate PPI networks could not truly represent the intracellular PPIs in zebrafish, we used interaction ability identification to determine the significant interactions. The refined intracellular PPI networks could thus be constructed from these significant interactions.