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Table 1 Definition of the different media used in this study

From: A curated C. difficile strain 630 metabolic network: prediction of essential targets and inhibitors

Medium name Componentse
Minimala Tryptophan, cysteine, isoleucine, leucine, methionine, proline, valine, R-pantothenate, Pyridoxine, biotin, glucose
BDMb Minimal plus thiamine, nicotinamide, Riboflavin, 4-aminobenzoate, folic acid, vitamin B12
CADMc BDM plus the 13 standard amino acids not listed in the minimal medium
Complexd All the components of the minimal medium, the remaining 13 standard amino acids, riboflavin, folic acid, inosine, vitamin B12, chorismate, glucose, glycerol-3-phosphate, ribose, hypoxanthine, deoxycytidine, uracil, uridine, dexoyadenosine, adenosine, thymidine
  1. a[36].
  2. b[37].
  3. c[38],[39].
  4. d[23].
  5. eThe following metabolites or proteins are added in SA analysis to permit the production of biomass: ATP, nicotinate, oxidized ferredoxin, oxidized thioredoxin, acyl and sulfur carrier proteins and are present in unchanging equilibrium concentrations in FBA.