VNG1237C acts as a repressor of 47 genes during growth in batch culture. A. and B. Expression of VNG1237C (dashed lines) and the median expression of its 45 target genes (solid lines) for two biological replicates (black and red) are strongly anti-correlated across the growth curve as measured through optical density at 600 nm (OD600) ,. Red arrows indicate the sampling OD600s of ~0.18 and ~1.15 used for microarray validations. C. Distribution of correlation coefficients for all 2,400 genes on the bottom (grey), all 2,400 genes in growth curve conditions (blue), 116 VNG1237C target genes in the growth curve conditions (red) and the 47 anti-correlated VNG1237C target genes in the growth curve conditions (purple). Red dashed line indicates median correlation coefficient of 116 VNG1237C target genes in the growth curve conditions used as a cutoff to discover 47 correlated VNG1237C target genes. D. Relative repression is significantly higher for the 47 VNG1237C target genes between △ura3 △VNG1237C and △ura3 control strain (p-value =0.05). ‘*’ means Wilcoxon rank-sum test p-value <0.05.