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Table 1 Characteristics of psd module variants

From: Photo-sensitive degron variants for tuning protein stability by light

Name Half-life in darkness (min) Half-life in 30 µmol m−2s−1blue light (min) Dark/light ratio of fluorescence measurements
Wild type psd module 123 ± 21 20 ± 1 10.8 ± 0.5
V19I 132 ± 50 17 ± 2 n.d.
K92R E132A E155G 102 ± 41 12 ± 0.4 21.7 ± 2.5*
K92R E132A N148D E155G 103 ± 26 10.5 ± 0.3 12.2 ± 1.1
K92R E132A E139N N148D E155G 66 ± 10 9.8 ± 0.4 16.3 ± 1.2*
K121M N128Y 44 ± 8 8.5 ± 0.3 14.3 ± 1.4*
K121M N128Y G138A 92 ± 28 13 ± 1 21.7 ± 4*
K121M N128Y N148E 87 ± 18 17 ± 2 9.5 ± 0.5
E132D E139K 89 ± 25 17 ± 3 n.d.
E137D 165 ± 43 24 ± 4 n.d.
E137D E151D 79 ± 25 21 ± 3 n.d.
G138A 147 ± 52 22 ± 1 n.d.
G138A V142A R154G E155S 42 ± 10 10.5 ± 0.8 14.4 ± 1.4*
G138A N148E 151 ± 53 75 ± 14 n.d.
G138A R154G E155S 27 ± 4 7 ± 0.3 n.d.
E139N 89 ± 22 11 ± 0.5 10.3 ± 0.4
V142G 31 ± 4 19 ± 3 n.d.
N148E 168 ± 53 39 ± 8 n.d.
N148E R154G E155S 91 ± 35 13 ± 1 13.5 ± 0.7*
E151D 103 ± 26 28 ± 6 n.d.
R154G E155S 14 ± 2 6.4 ± 0.2 n.d.
Δ L156 Δ P157 187 ± 45 52 ± 9 n.d.
degODC 86 ± 21 20 ± 3 n.d.
CACA 87 ± 21 20 ± 3 n.d.
CACACA 66 ± 13 16 ± 1 n.d.
  1. The half-lives were obtained by cycloheximide chase analysis and the ratio by fluorescence intensity measurements of cells kept in darkness or exposed to blue light (30 µmolm − 2 s− 1). The asterisk (*) marks dark/light ratios that are considered to be statistically different from the ratio of psd (P <0.05).