PCA shows differences between environmental perturbations at late time points for OCCs and OCSCs. PCA shows separation between conditions and time points at 24 and 48 hours. In both cell types, PC1 plays a large role in separating time points, and PC2 captures variation between the conditions. Dotted ovals represent 95% confidence intervals of the membership of each sample class. A) Control samples show little temporal variation in OCCs. Effects of ischemia are not additive based on the effects of glucose deprivation and hypoxia individually at 48 hours. B) OCSC control samples display much greater temporal variation compared to OCCs. There is no metabolic distinction between control and hypoxic cells, and 48-hour ischemia samples again show non-additive effects compared to glucose-deprived and hypoxic conditions.